Part:BBa_K133008:Design

CF213-UB33

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 718
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 301
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is an intermediate to other parts: [1] and [2]

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).

Source

plasmid DNA (CF213 was amplified with PCR from chimeric flagellin, multiepitope was ordered from GeneArt)